Effect of acutobin on the activity of fibrinolysis in cultured human umbilical vein endothelial cells / 中国药理学通报

ABSTRACT

AIM This study is to observe the effects of acutobin on the activity of tissue type plasminogen activitor(t PA) and tissue plasminogen activitor inhibitor(PAI) in the cultured human umbilical vein endothelial cells, aiming at disclosing some of the mechanisms of thrombolysis of acutobin. METHODS Endothelial cells were isolated from fresh human umbilical cords by trypsin digestion of the interior surface of the umbilical vein. Cultured cells were examined by light, phase contrast and electron microscopy. The factorⅧ related antigen and CD34 of the cells were detected by AEC and DAB staining. Chromogenic assay was used to identify the activity of t PA and PAI in the medium of culture cells. Fibrin degradation products(FDPs) were measured using ELISA kit. RESULTS The cultured human umbilical endothelial cells were shown as monolayers of closely opposed, polygonal cobblestone shape by light and phase contrast microscopy. By transmission electron microscopy, cultured endothelial cells contained Weibel Palade body and showed tight junction with each other. The cells contained abundant quantities of CD34 and factorⅧ related antigen. The intercellular space among individual cell enlarged and lost polygonal cobblestone shape in the present of acutobin. Activity of t PA increased, the activity of PAI did not change significantly and FDPs increased significantly in the culture medium. CONCLUSIONS The study demonstrates the culture cells was endothelial cells according to morphologic and immunohistologic criteria. Acutobin increases the fibrinolytic activity of cultured endothelial cells and may exhibit antithrombotic effect in vivo.