Effect of electrical stimulation to cerebellar fastigial nucleus on the expressions of PKC? and PKC? in the somatosensery cortex and basal ganglia in rats / 第三军医大学学报

ABSTRACT

Objective To investigate the mechanism of preconditioned neuroprotection of cerebellar fastigial nucleus electrical stimulation(FNS) by means of observation of the effect of FNS on the expressions of PKC? and PKC? in the somatosensery cortex and basal ganglia in rats. Methods FNS models in rats were established. Brain tissue containing the somatosensery cortex and basal ganglia was removed at 0 h and 1, 3, 7 and 10 d respectively after FNS and 20 ?m coronal sections were obtained with a sliding cryo microtome. Immunocytochemical analysis of PKC? and PKC? in 2 sections from each rat was performed and the average optical density of immunostaining was quantified by computer assisted image analysis system. Sham stimulation rats and cerebral dentate nucleus (DN) stimulation rats were used as the control. Results At 0 h after FNS, no significant changes of the expressions of PKC? and PKC? were found in the contralateral somatosensery cortex and basal ganglia. The expressions of PKC? and PKC? increased significantly at 1 d and decreased at 3 d but were still higher than those in sham stimulation group at 7 d and decreased to the basal level of the control at 10 d. However, the expressions of PKC? and PKC? in the ipsilateral somatosensery cortex and basal ganglia were also elevated at 1 d after FNS but the increase was significantly lower than that in the contralateral ones and reached the basal level at 3 d. There was no change of PKC? and PKC? in sham stimulation group at 1 d after DN stimulation. Conclusion The increased expressions of PKC? and PKC? in the somatosensery cortex and basal ganglia induced by FNS may participate in the preconditioned neuroprotection of FNS.