An experimental study of glucose transporter 1 protein expression in the burned rat liver / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Article
in Chinese
| WPRIM
| ID: wpr-678542
ABSTRACT
Objective To investigate the expression of glucose transporter 1(GLUT 1) and its transcription activity in the liver of burned rats Methods The Wistar rats inflicted with 30% TBSA full thickness flame burn on the back were sacrificed at 0 5, 1, 2, 4, 8 and 16 h after burn GLUT 1 protein levels in the rat livers were determined by Western Blotting with the reference to those in 6 normal rats The liver cells were transfected with Construct A, and 24 h later subjected to hypoxia (1%O 2) to mimic the hypoxia environment The samples were harvested at 3, 6 and 12 h and determination of the reporter gene luciferase and pSV ? galactosidase activities was performed Results ①Compared with that in the normal control, GLUT 1 protein level in the liver was significantly increased( P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Third Military Medical University
Year:
2003
Type:
Article
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