Effect of c-fos antisense gene transfection on protection of cardiomyocytes following hypoxia and burn serum treatment in rats / 第三军医大学学报

ABSTRACT

Objective To explore the effect of c fos antisense gene transfection on the protection of cardiomyocytes following hypoxia and burn serum treatment. Methods Burn serum was collected from Wistar rats with 30% total body surface area(TBSA) of Ⅲ degree burns. The mixture gas containing 1% O 2 was used as hypoxia model. The c fos antisense gene recombinant was constructed by genetic recombination technique. Cardiomyocytes from neonatal Wistar rats were cultured in vitro with hypoxia and burn serum treatment. c fos antisense gene recombinant was transfected into the cultured cardiomyocytes. Expression of c fos mRNA was determined by RT PCR. Expressions of c fos protein, troponin T and ? Tubulin in cardiomyocytes were determined by Western blotting in the transfected and non transfected groups. Results RT PCR results showed that the expression of c fos mRNA increased significantly in the non transfected group. But after transfection of c fos antisense gene recombinant, the expression of c fos mRNA decreased significantly as compared with the non transfected cardiomyocytes. Western blotting results showed that the expression of c fos protein in the transfected group decreased remarkably as compared with the non transfected group, but the expressions of ? Tubulin and troponin T increased significantly in the transfected group. Conclusion Burn serum and hypoxia can cause the injury of cardiomyocytes. c fos antisense gene recombinant transfection has the protective effect on cardiomyocytes exposed to burn serum and hypoxia.