Protective effects of diazoxide on cardiomyocytes after severe burn injury and the related mechanism / 第三军医大学学报

ABSTRACT

Objective To investigate the protective effects of diazoxide on cardiomyocytes after severe burn injury Methods A total of 24 healthy Wistar rats were randomized into normal control group(Control), burn group(Burn) and diazoxide treated group(Diazo)( n =8) Rats in Burn and Diazo groups were inflicted with 30%TBSA Ⅲ degree burn and resuscitated with Ringer's solution intraperitoneally 30 min after burn Diazoxide was injected into rats in Diazo group at the dose of 10 mg/kg through the external jugular vein After rats were sacrificed at 6 h after burn, myocardial mitochondrial K + influx, respiratory function, Ca 2+ concentration ([Ca 2+ ]m), MDA content, serum CK and LDH levels were determined Results Mitochondrial K + influx of Diazo group was evidently higher than that in Control and Burn group Mitochondrial respiratory control rate(RCR) and ST 3 in Diazo group were higher than that in Burn group However, [Ca 2+ ]m, MDA, CK and LDH levels in Diazo group were significantly lower than those in Burn group Conclusion Diazoxide can attenuate the damage to cardiomyocytes after severe burn injury, which might be related to the opening of mitochondrial K + channel, inhibition of mitochondria from Ca 2+ overloading and decrease of free radical production