Expression and significance of LBP mRNA in early liver tissue after burn combined with endotoxemia in rats / 第三军医大学学报

ABSTRACT

Objective To explore the pathogenesis of early liver damage after burn combined with endotoxemia in rats. Methods A total of 156 healthy Wistar rats were randomized into burn combined with injection of endotoxin group (BCEG), single burn group (SBG), single endotoxin injection group (SEG), and normal control group (NCG). An animal model of rats inflicted by 20% TBSA Ⅲ degree burn combined with intraperitoneal injection of lipopolysaccharide (LPS) was used for this experiment. The changes of liver morphology, the hepatic functions, and the localization of liver LBP mRNA were determined by light microscopy and electron microscopy, quantitative analysis, and in situ hybridization(ISH). Results More serious damages in BCEG were found than those in SBG and SEG. ALT activity in BCEG increased significantly at 0 5 h ( P