Culture of osteoblasts by serial explant culture and comparison of their characteristics / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6)2004.
Article
in Chinese
| WPRIM
| ID: wpr-678755
ABSTRACT
Objective:
To Investigate a simple, economical and efficient method of primary osteoblast culture and compare their characteristics.Methods:
Primary osteoblasts of 1 st -4 th series explant culture from calvarial bones of neonatal Sprague Dawley rats,were collected and osteoblasts shape, mitosis, proliferation, ALP activity and immunohistological expression of osteocalcin and BMP 2 observed.Results:
No difference was found in cell shape, spread, conflunce, ALP activity as well as immunohistological stain of osteocalcin and BMP 2 of the osteoblasts, which had been harvested from the 1 st -4 th series explant cultures of Sprague Dawley rats.Conclusion:
Series explant culture can harvest the same kind of osteoblasts as the explant culture, and more osteoblasts can be obtained at a single time. It saves money and time and is easy to manipulate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Peking University(Health Sciences)
Year:
2004
Type:
Article
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