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Cellular mechanism of renal proximal tubular epithelial cell injury induced by aristolochic acid Ⅰ and aristololactam Ⅰ / 北京大学学报(医学版)
Journal of Peking University(Health Sciences) ; (6)2004.
Article in Chinese | WPRIM | ID: wpr-678764
ABSTRACT

Objective:

To investigate the cellular mechanism of renal proximal tubular epithelial cell(PTEC) injury induced by aristolochic acid Ⅰ (AA Ⅰ) and aristololactam Ⅰ (AL Ⅰ).

Methods:

Human PTEC cell line HK 2 was used as the subject. Cell apoptosis was evaluated by using FACS. Fibronectin (FN) and TGF ?1 levels were assayed in the supernatant from cultured HK 2 cells by ELISA. In the blocking study, anti TGF ?1 neutralizing antibody was used as an antagonist. The changes of FN level and apoptosis were compared.

Results:

After stimulation by 2.5 mg/L AA Ⅰ, HK 2 cells secreted TGF ?1 at hour 12 and FN at hour 36. HK 2 cell apoptosis was detected at hour 48. Anti TGF ?1 neutralizing antibody (5 mg/L) could suppress AA Ⅰ induced apoptosis by 63.7% ( P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Peking University(Health Sciences) Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Peking University(Health Sciences) Year: 2004 Type: Article