Protective effect of GSTT on myocardium injuried by ischemia reperfusion / 中国药理学通报

ABSTRACT

AIM To investigate the protective effect of GSTT on myocardial injury induced by ischemia reperfusion. METHODS The content of lactic dehydrogenase(LDH),creatine kinase(CK),methylenedioxyamphetamine(MDA),superoxide dismutase(SOD),tumor necrosis factor alpha(TNF ?) and interleukine 6(IL 6) were determined in medium after reperfusion by simulating model of ischemia reperfusion; The content of nitric oxide(NO) was determined in medium by TNF ? injury myocardium,and mitochondrial activity was determined by MTT after TNF ? stimulated. RESULTS Compared with the model control,in the GSTT, the content of LDH,CK,MDA,TNF ? and IL 6 in medium decreased and activity of SOD increased after reperfusoin,the level of NO in medium decreased and OD 490 increased after TNF ? injuried. CONCLUSION It is suggested that GSTT can mitigate injury of ischemia reperfusion on myocardium, and has a direct protective effect on injuried myocardium.