Changes and significance of P~(53) , C-myc, Bax, and Bcl-2 in surviving HepG_2 cells under continuous stress of cisplatin / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2002.
Article
in Chinese
| WPRIM
| ID: wpr-678986
ABSTRACT
Objective To observe the dynamic changes of P 53 , C myc, Bax, Bcl 2, and NF ?B in surviving HepG 2 cells under the continuous stress of cisplatin for the investigation of the potential role in the resistance of HepG 2 cells to cisplatin. Methods The apoptosis of HepG 2 cells and changes of the apoptosis associated factors including P 53 , C myc, Bax, Bcl 2, and NF ?B in the surviving HepG 2 cells were detected by immunohistochemical and RT PCR assays. Results No obvious change of P 53 and C myc was found in the surviving HepG 2 cells under cisplatin stress, but persistent increase of Bcl 2 and decrease of Bax and a continuous increase of Bcl 2/Bax ratio were found. Markedly positive expression of NF ?B mRNA was found at 7 d after the stress of cisplatin. Conclusion The mechanisms of the surviving HepG 2 cells under continuous stress of cisplatin may be associated with the increase in Bcl 2 and decrease in Bax, specially the continuous increase in Bcl 2/Bax ratio, while the positive NF ?B mRNA expression may be associated with the later events after HepG 2 cell survive.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Third Military Medical University
Year:
2002
Type:
Article
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