Inhibitory effect of arsenic trioxide on survivin expression in heterologous graft model of human breast infiltrating duct carcinoma in nude mice / 第三军医大学学报

ABSTRACT

Objective To investigate the inhibitory effect of arsenic trioxide (As 2O 3) on the survivin expression in heterologous graft model of human breast infiltrating duct carcinoma in nude mice. Methods Nude mice bearing the transplanted tumor were randomly divided into four groups negative control (saline), positive control (cisplatin at the dose of 1.5 mg/kg), and two experimental groups (arsenic trioxide at the doses of 1.5 mg/kg and 3.0 mg/kg). The expression of survivin mRNA and protein expressions of surviving and Caspase 3 in neoplastic tissues were detected by in situ hybridization and immunohistochemical method, respectively. Results In negative control group, the expressions of survivin mRNA and survivin protein were in moderate degree with positive rates of 82.42% and 78.85%, respectively. The positive rates of moderate degree in experimental groups 1 and 2 were gradually decreased with the increasing dose of arsenic trioxide ( P