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Effects of serum of pregnancy-induced hypertension on umbilical vein endothelial cells / 第三军医大学学报
Article in Zh | WPRIM | ID: wpr-679092
Responsible library: WPRO
ABSTRACT
Objective To observe the changes in secretory functions of cultured umbilical vein endothelial cells injured by serum from pregnancy induced hypertension (PIH). Methods Endothelial cells isolated from the umbilical veins from moderate or severe PIH women (PIH group, n =10) and normal pregnant women (control group, n =8) were incubated at 37 ℃ in humidified air with 5% CO 2. Cell culture fluids were obtained at 3 and 4 d of primary culture. Contents of nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), lactate dehydrogenase (LDH ) in the cell culture fluids were measured. Results LDH activity and MDA contents in the cell culture fluids in PIH group were significantly higher than those in the control group ( P
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Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2003 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2003 Type: Article