Effects of nimesulide on the proliferation and the expression of E-cadherin of human colonic carcinoma cell lines / 第三军医大学学报

ABSTRACT

Objective To evaluate the potential roles of nimesulide, a nonsteroidal anti inflammatory drug (NSAID), on the proliferation and the expression of E cadherin of human colon adenocarcinoma cell lines. Methods The human colon cancer cell lines HT 29 and HCT 116 were employed in the study. After treatment with nimesulide, the inhibitory effect of nimesulide on the proliferation of cancer cells was quantified by MTT assay, and the expression of E cadherin in the cells was detected by immunohistochemical streptavidin/peroxidase method. Results Nimesulide inhibited the proliferation of HT 29 and HCT 116 cells in time and dose dependent manners. The inhibitory effect on HT 29 cells was stronger than that on HCT 116 cells. Nimesulide up regulated the E cadherin expression of HT 29 cells, whereas the expression level of HCT 116 cells remained unchanged. Conclusion Nimesulide can obviously inhibit the growth of colon cancer cells HT 29 overexpressing cyclooxygense 2 protein, suggesting that nimesulide may up regulate the expression of E cadherin through the inhibition of COX 2 activity, resulting in decrease of the invasion and metastatic ability of tumor cells.