The Isolation,Culturing and Differentiation of Neural Stem Cells Originated from Rat Embryonic Brain / 医学研究杂志

ABSTRACT

Objective To investigate the met ho ds of isolation,culturing and passaging of neural stem cells originated from ra t embryonic brains.Methods The neural stem cells were obtained from the brain tissue of rat embryos.They were cultured and passaged with serum-free medium.T hey were identified with Nestin and stained with NF68,GFAP,and GalC after diff erentiation.Results We cultured the neural stem cells through several passages .With the help of serum,they differentiated to neurons,astrocytes and oligode ndrocytes.Conclusion The neural stem cells derived from rat embryonic brains a re able to proliferate and multiplepotent for differentiation.