Expression and Purification of the Fusion Protein Anti-epilepsy Peptide/Glutathiones-transferase / 浙江中医药大学学报
Journal of Zhejiang Chinese Medical University
;
(6)2007.
Article
in Chinese
| WPRIM
| ID: wpr-679634
ABSTRACT
[Aim] To gain the fusion protein purified GST-AEP.[Methods] The protein GST-AEP was expressed in E.Coli-DH5? as a fusion protein induced by IPTG.The protein was a kind of inclusion body.The purifying and refolding to inclusion body were optimized.The purity of GST-AEP was identified by 12% SDS-PAGE and thin-layer scanning analysis.The quantitation of the fusion protein GST-AEP was done with BCA Protein Assay.[Results] Purity of GST-AEP was higher than 90% and concentration was about 0.163?g/?l.[Conclusion] The fusion protein was highly purified and the method of fusion protein purification from the inclusion body was developed,which was the basis for further study on AEP.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Zhejiang Chinese Medical University
Year:
2007
Type:
Article
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