APPLICATION OF COLLOIDAL GOLD-TECHNIQUE FOR LOCALIZATION OF CON A RECEPTORS IN HUMAN SPERM / 解剖学报

ABSTRACT

Intact and gossypol-treated spermatozoa from healthy man were labeled withCon A-HRP-G two-step method.Comparative observations of these sperms were doneunder LM,TEM and SEM.Results show that there are gold particles on spermsurface membrane.Their distribution is more dens on plasma membrane of spermhead and middle piece.On the intracellular membrane structures of sperm which plasmamembrane was damaged by gossypol the gold particles also can be seen,especially onthe membranes of acrosome and mitochondria.It was proved that Con A receptorsare present not only on plasma membrane of sperm,but also on the membrane ofacrosome and mitochondrion.The significance of Con A receptors on sperm struc-tures,advantages of Con A-HRP-G two-step labeled method and comparativeobservations under LM,TEM and SEM were discussed.