CYTOCHEMICAL OBSERVATION OF THE EPIMERASE ACTIVITIES OF BONE MARROW STROMAL CELLS IN CULTURE / 解剖学报

ABSTRACT

Fibroblasts, reticular cells and macrophages are the important components of bone marrow hemopoietic in liquid to study whether HP-Ⅱ-E(Hydroxyproli- ne-2-epimerase, EC5, 1, 1,a) is involved in the synthesis of functional proteins of stromal cells. The femoral bone of 6 to 8 week-old 615 mice were made in suspension for cell culture in vitro. DMEM medium supplied with 25% calf serum was used After one to fifteen day's attaching incubation in stationary liquid the culture cells were stained with Giemsa, nonspecific esterase and HP-Ⅱ-E at different intervals. From the third day of the incubation, reticular cells began to develop form- ing stallate cells of the bone marrow. During an earlier time, enzymatic reaction was only observed in the nuclear membrance, but after three day's culture all the enzymatic activity in the cells increased dramatically. The enzymatic reac- tive granules were abundant in the outer cytoplasma of the stallate cells and fibroblasts and the cytoplasma of the macrophages. It was observed that hyd- roxyproline was changed by epimerase,