Determination of Atractylenolide Ⅰ in Xiangshayangwei Pills by RP-HPLC / 中成药

ABSTRACT

Objective: To develop a method to determine the content of a atractylenolideⅠ in Xiangshayangwei Pills. Methods: Reverse Phase HPLC was used to determine the content of atractylenolideⅠ in Xiangshayangwei Pills. The separation was performed on YWG ODS column with methol water (60∶40) as a mobile phase and the wavelength of UV detector was 220nm. Results: The linearity of this method was well with the average recovery of 99.54%. Conclusion: The method is simple, reliable, and sensitive. It also shows good separating degree. It can be used in quality controll of Xiangshayangwei Pills.