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Determination of Cinobufagin and Resibufogenin in Qianglijiuxin Dripping Pills by HPLC / 中成药
Chinese Traditional Patent Medicine ; (12)1992.
Article in Chinese | WPRIM | ID: wpr-681218
ABSTRACT

Objective:

To determine the contents of cinobufagin and resibufogenin in Qianglijiuxin Dripping Pills.

Methods:

The HPLC method was used at Kromasil C 18 column of 250mm?4.6mm, 5?m, 0.5% Dipotassium Hydrogen Phosphate Acetonitrile (50∶50) as the mobile phase, 1.0mL/min as the flow rate at detection wavelength of 296nm.

Results:

For cinobufagin, the linear range was 0.67?g~4.7?g and the average recovery rate was 97.78% with RSD=1.2%. For resibufogenin, the linear range was 0.33?g~2.3?g and the average recovery was rate 99.61% with RSD=2.0%.

Conclusion:

The method can be applied to content determination of cinobafagin and Resibufogenin in Qianglijiuxin Dripping pills.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional Patent Medicine Year: 1992 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional Patent Medicine Year: 1992 Type: Article