Determination of protopine and tetrahydropalmatine in Corydalis Decumbens (Thunb.) Pers. by HPLC / 中成药

ABSTRACT

Objective:To establish the content determination method of the main alkaloids protopine and tetrahydropalmatine in Corydalis decumbens (Thunb.) Pers. Methods: HPLC was used to determinate the main alkaloids protopine and tetrahydropalmatine in Corydalis decumbene (Thunb.) Pers. Supelcosil LC 18 DB Column was used, mobile phase consisted of Methol NaAc/HAc(75∶25 pH5.0) and detection wavelength at UV 285 nm. Results: Linearity of this method was good with the average recoveries, 98.04% and 99.63%. Conclusion: This method is accurate, reliable with good separability and reproducibility, it can be applied as standard for the control of Corydalis decumbens (Thunb.) Pers.