Determination of the contents of glycyrrhizine and paeoniflorin in Fubao Danggui Jiao by RP-HPLC / 中成药

ABSTRACT

Objective: A method for determination of glycyrrhizine and paeoniflorin in Fubao Danggui Jiao by RP HPLC was established.Methods: Shim pack CLC ODS column was used with TBAH solution (10→300) methanol (36∶64) (pH=6.0) as a mobile phase for glycyrrhizine and acetonitrile water (containing 0.1% phosphoric acid) (15∶85) as a mobile phase for paeoniflorin. The detection wavelength was at 254nm and 230nm, respectively. The flow rate was 1mL?min -1 .Results: A good linearity was found at the concentration range of 100 1000ng for glycyrrhizine and paeoniflorin. The average recovery was 98.37%,97.44% and the relative standard deviation was 0.32%,0.48%, respectively.Conclusion: This method was simple,accurate,rapid,sensitive and reproducible. The RP HPLC could be used for quality control of Fubao Danggui Jiao preparations.