Determination of cinobufagin and resibufogenin in Shexiangbaoxin Pill by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-681869
ABSTRACT
Objective:
To establish the determination of Shexianbaixin Pill.Methods:
HPLC was applied to determine cinobufagin and resibufogenin. The determination was carried out using an ODS column with a solvent system acetonitrile 0.5KH 2PO 4. The flow rate was 1.0mL?min -1 , detection wavelength was at 296nm and the column temperature was at 35℃.Results:
The linear ranges of cinobufagin and resibufogenin were 0.148?g~0.746?g and 0.116?g~0.580?g, respectively. The average recoveries of cinobufagin and resibufogenin were 100.90%( RSD =1.004%, n =5), and 100.64%( RSD =1.006%, n =5), respectively.Conclusion:
This method is simple, accurate with strong specificity and can be used to control the quality of Shexiangbaoxin Pill.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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