HPLC separation and determination of bufadienolide in Cinobufacini Injection / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-681914
ABSTRACT
Objective:
To determinate the content of bufalin,resibufogenin and cinobufagin in Cinobufacini Injection.Methods:
Liposoluble components in cinobufacini were extracted with ethyl acetate and determined by HPLC using a C 18 column, acetonitrile water(50∶50) as a mobile phase and UV detection wavelength at 299nm.Results:
The method showed the good resolution, high sensitivity, satisfactory accuracy and specificity. Quantitatively analyze results showed that the concentration of bufalin, cinobufagin and resibufogenin in Cinobufacini Injection were 0.333 ?g?mL -1 , 0.159?g?mL -1 and 0.110?g?mL -1 , respectively.Conclusion:
Bufalin in Cinobufacini Injection reached effective concentration, which was regarded as one of anti cancer components.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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