Determination of diosgenin in Rhizoma Paridis by HPLC and TLC / 中草药

ABSTRACT

Object To determine the contents of diosgenin in Rhizoma Paridis by HPLC and TLC. Methods ODS column was used in HPLC with mobile phase acetonitrile water(90∶10), detection wavelength 203 nm, column temperature 35 ℃; silica gel plate was used in TLC with developer cyclohexane ethyl acetate (4∶1), scan wavelength 610 nm, reference wavelength 420 nm. Results The linear ranges of HPLC and TLC were 1 2 - 7 2 ?g (r=0 999 8), 0 2 - 1 0 ?g (r=0 995 7). The average recovery and RSD were 102 2%, 3 39% (n=6) and 100 9%, 2 68% (n=6) respectively. Conclusion Both of two methods can be used for the quality control of diosgenin in Rhizoma Paridis.