Effect of curcumin on primary cultured rat hepatocyte injurid by CCl_4 / 中成药

ABSTRACT

Objective: To investigate the protective effect of curcumin on carbon tetrachloridlde (CCl 4) intoxicated primary cultured rat hepatocytes. Methods:The rat hepatocytes were separated by perfusion with collagenase solution. Curcumin was added to the culture medium after incubating the rat hepatocyte for 36 hours. At the same time, CCl 4 was added to induce hepatocyte injury for 1 h. The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined, respectively. Results:Curcumin significantly improved cell viability, inhibited the decrease of SOD activity and increase of the ALT, LDH, MDA, NO levels. Conclusion: Curcumin protected effectively the primary cultured rat hepatocytes against injury induced by CCl 4.