Determination of rehmannioside D in root ofRehmannia glutinosa by HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24)1994.
Article
in Chinese
| WPRIM
| ID: wpr-682137
ABSTRACT
Object To establish an HPLC method for the determination of rehmannioside D in the root of Rehmanniae glutinosa (Gaert.) Libosch. ex Fisch et Mey and provide a limitation for the lowest content of rehmannioside D Methods Chromatographic conditions Hypersil C 18 (200 mm?4.6 mm, 5 ?m) column, mobile phase acetonitrile water (4∶96), flow rate 1 mL/min, room temperature, detection wavelength 205 nm Results The recovery was 100.6%, and RSD was 2.1% for rehmannioside D. From eight different regions 16 samples of R glutinosa were quantitatively analysed and the contents of rehmannioside D in R glutinosa were 0.137%-0.691% Conclusion The method is rapid, simple and accurate, and can be used for the quality control of R glutinosa The proposed content of rehmannioside D must be no less than 0.15%.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
1994
Type:
Article
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