Isolation and identification of active components of Ganoderma lucidum cultivated with grass and wood log I. Extraction, purification and characterization of glycopeptide / 中草药

ABSTRACT

Object To study the amount and properties of glycopeptide of Ganoderma lucidum (Leyss ex Fr ) Kaist. cultivated with grass (GLPG) and wood log (GLPW) ; to investigate the feasibility of substitution of grass for wood log to cultivate G lucidum Methods Glycopeptides were extracted and isolated from the fruit body of G lucidum followed by dialysis; the monosaccharid constituent and relative molecular weight of polysaccharides were examined by GC and gel filtration; structure of glycopeptide was determined by IR, 1H NMR and 13 C NMR Results Relative molecular weights of GLPW and GLPG glycopeptides were (Mw) 5 13?10 5 and 5 85?10 5, (M?) 6 21?10 5 and 6 96?10 5, respectively; the glucosidic bonds of both glycopeptides were ? type; both glycopeptides consist 16 amino acids and Rha, Xyl, Fru, Gal and Glc (there was trace amount of Man in GLPW) Conclusion There are less physical and chemical difference between GLPG and GLPW The purification rate of GLPG is 2 8 times to GLPW