INTERRELATION BETWEEN MICROTUBULE-ASSOCIATED PROTEIN 5 AND LYSOSOME IN CULTURED SPINAL NEURONS FROM THE RAT / 解剖学报

ABSTRACT

Objective To investigate the interrelation between microtubule associated protein 5 and lysosome in cultured spinal neurons. Methods Neuron culture, immunofluorescence cytochemistry, laser confocal scanning microscope were used. Results In cultured spinal neurons, both of MAP5 and calthepsin D were distributed in cytoplasm and processes. In merged pictures, the distribution of MAP5 and calthepsin D was almost similar. When nocodazole was employed, the distribution of MAP5 and calthepsin D changed simultaneously. Both fluorescence distributed in cytoplasm was uneven and the process was shorter. When PMA was employed, the distribution of red and green fluorescence became reticulum and spread toward membrane. The soma enlarged and the process displayed certain direction accompanying adjacent processes interlinked.Conclusion In cultured spinal neurons, the distributions of MAP5 and lysosomes were almost similar. Both were in cytoplasm and process. Nocodazole and PMA caused reverse change for the distribution of MAP5 and lysosomes. The distribution of MAP5 and calthepsin D treatel with Nocodazole was unregulated, while PMA was regulated and loose. These results suggested that MAP5 and lysosomes were interrelated between structure and function. Both of them were depended on the integrity of microtubule.