The value of cDNA microarray technology in the studies on the changes of gene expression profiles in gastric carcinoma cells induced by parvovirus H-1 infection / 中华消化杂志

ABSTRACT

Objective To investigate the role of cDNA microarray techniques in detection of the changes of gene expressional profiles in the gastric carcinoma cell line HGC27 induced by parvovirus H 1 infection. Methods The mRNA of gastric carcinoma cell line HGC27 was collected at the time before or 48 h after H 1 virus infection. Using cDNA microarray, we investigated the diversification of gene expression of HGC27 after H 1 virus infection. A part of genes whose expressions were changed in cDNA microarray analysis were further identified by RT PCR and Northern blot. Results The cDNA microarray analysis showed that the expressions of 920 genes were changed among total of 8000 genes, in which 363 genes showed decreased expressions whilst 557 gene expressions were increased. Among them, some of the genes, including ? raf, sarp1, creb, p38 ? and rad21, were further confirmed by RT PCR and Northern blot analysis. The results of RT PCR and Northern blot were coincided well with the cDNA microarray results. Conclusions The cDNA microarray is an effective method for the large scale comparison of multiple genes expressions in a single hybridization and for the studies on anti tumor mechanisms of parvovirus H 1.