RP-HPLC determination of cinobufagin and resibufogenin in Niuhuangxiaoyan Tablet / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-682502
ABSTRACT
AIM:
To estalish a HPLC method for the determination of cinobufagin and resibufogenin in Niuhuangxiaoyan Tablet(Calculus Bovls, Radix et Rhizoma Rhei, Concha Margaritifera Usta, Venenum Bufonis, etc.).METHODS:
Waters Symmetry Shield RP18(3.9mm?250mm,5?m) column at 30℃ was used with a mobile phase consisted of acetonitrile -0.5mol?L -1 potassium dihydrogen phosphate(solution was adjusted by phosphoric acid to pH=3.2) (42∶58) and a UV detector at 296nm. The flow rate was 1.0mL?min -1 .RESULTS:
The linear range of cinobufagin was 0.0696~0.8352?g. The RSD of measurement precision test was 0.53%; The average recovery was 100.06% ( RSD =1.25%, n =5). The linear range of resibufogenin was 0.1576~0.9456?g. The RSD of measurement precision test was 0.15%. The average recovery was 100.54%( RSD =1.49%, n =5).CONCLUSION:
The method is simple and accurate, and can be used for the quality control of Niuhuangxiaoyan Tablet.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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