Optimization technique in separation of the total flavonoid from Radix Puerariae with macroporous resin / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-682506
ABSTRACT
AIM:
To explore the optimal separation of the total flavonoid from Radix Puerariae by selecting appropriate macroporous resins and to study systematically the factors which affect separation.METHODS:
Static and dynamic adsoption desorption methods were adopted, and evaluated for separating efficiency by measuring the concentration of total flavonoid in Radix Pueraria with UV spectrophotometer.RESULTS:
The SP70 macroporous resin had the best separating efficiency when the flavonoid content in the liquid was 0.5g per 1mL equivalemt to raw material. pH5~6. the volume of drug 60BV (resin bed volume) with the adsorption power 2BV/h. and the volume of 70%(mL?mL -1 ) ethanol as eluant 4BV with desorption power 2BV/h. In result. total flavonoid's purity could reach 80%.CONCLUSION:
This method is simple and feasible with good effect of separation, which can meet industrial requirements.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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