Role of matrix metalloproteinase-2, 9 and their inhibitors in premature rupture of membranes / 中华妇产科杂志

ABSTRACT

Objective To investigate the role of matrix metalloproteinase 2, 9 (MMP 2, MMP 9) and tissue inhibitor of matrix metalloproteinases 2, 1(TIMP 2, TIMP 1 ) in human amniochorionic membrane Methods Amniochorionic membranes were collected from the following groups of women (1) women with spontaneous premature rupture of membrane (PROM) before onset of labor (PROM group, n =8), (2) Women with term labor after vaginal delivery (vaginal delivery group, n =8), (3) Women undergoing elective cesarean section (C section group, n =8) Messenger ribonucleic acid expression for MMP 2, MMP 9, and their specific inhibitors TIMP 2 and TIMP 1 were studied with reverse transcriptase polymerase chain reaction Results (1)MMP 2 level in PROM group was 0 849?0 037;in vaginal delivery group 0 327?0 023;in C section group 0 307?0 028 Expression in PROM group was highest, with significant difference compared with the other groups ( P 0 05) (2)MMP 9 level in PROM group was 0 026?0 004;in vaginal delivery group 0 008?0 001,with significant difference between the two groups ( P 0 05) Conclusions In PROM group,the expression of MMP 2, MMP 9 and their inhibitors TIMP 2, TIMP 1 is imbalanced, leading to increased extracellular matrix degradation, and weakening of the fetal membranes, and eventually premature rupture of the membranes