Local immune response in gastric mucosa by immunization of H.pylori vaccine with chitosa as adjuvant / 中华消化杂志
Chinese Journal of Digestion
; (12)2001.
Article
in Zh
| WPRIM
| ID: wpr-682620
Responsible library:
WPRO
ABSTRACT
Objective To study the adjuvant effects of chitosan on Helicobacter pylori (H.pylori) vaccine.Methods A total of 20 adult mice were randomly divided into four groups.Mice in groups Ⅰ,Ⅱ,Ⅲ, and Ⅳ were orally immunized with PBS,H.pylori antigen alone,H.pylori antigen plus chitosan solution or H. pylori antigen plus chitosan particles,respectively.An ELISA was used to detect anti-H.pylori IgA in saliva and gastric mucosa ,interlukin(IL)-2,IL-4,IL-10 levels in gastric mucosa.Immunohistochemical method was used to detect secretory IgA in gastric mucosa.Results ①The levels of special anti-H.pylori IgA in saliva and gastric mucosa in the groups with chitosan as adjuvant were significantly higher than those in the group without adjuvants and control groups(P
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Digestion
Year:
2001
Type:
Article