Changes and clinical significance of serum cytokines in patients with bee-sting poisoning / 中华急诊医学杂志

ABSTRACT

Objective To investigate changes and clinical significance of serum granulocyte-macrophage colony stimulating factor(GM-CSF),tumor necrosis factor-alpha(TNF-?),interleukin-6(IL-6)and interleukin-8(IL-8)level in patients with bee-sting poisoning.Methods One hundred and nine patients with bee-sting poisoning were also divided into slight poisoning group,moderate poisoning group,and server poisoning group and also divided into dead group and survival group according to the prognosis.Thirty healthy persons were selected as control group.GM-CSF and TNF-?were measured using radioimmunoassay,IL-6 and IL-8 was measured using enzyme linked immunoadsorbent assay(ELISA),and they were compared with control grouup.Results The levels of serum GM-CSF,TNF-?,IL-6,IL-8 in slight poisoning group were increased a little,and there were no significant difference compared with control group(P＞0. 05).The levels of serum GM-CSF,TNF-?,IL-6 and IL-8 were significantly higher in moderate poisoning group and severe poisoning group than those in the control group(P＜0.01),but the severe poisoning group were much higher than the moderate poisoning group(P＜0.01).They were significantly higher in survival group than those in dead group(P＜0.01).APACHEⅡscores were obvious correlated with the levels of serum GM-CSF,TNF-?,IL-6 and IL-8(P＜0.05 or P＜0.01). Conclusion GM-CSF,TNF-?,IL-6 and IL-8 may participate in the occurrence and progress of patients with bee-sting poisoning.Dynamic monitoring of serum GM-CSF,TNF-?,IL-6,IL-8 and APACHEⅡscores plays an important role in diagnosis of the severity of patients with bee-sting poisoning and its prognosis.The use of antagonist and inhibitor of cytokines may be helpful to treat patients with bee-sting poisoning.