Association between rheumatoid arthritis and shared epitope of HLA-DRB1 gene in Han nationality Neimenggu population / 中华风湿病学杂志

ABSTRACT

Objective To investigate the assoiation between rheumatoid arthritis(RA)and the pres- ence of the shared epitope(SE)of HLA-DRBI gene in Han nationality of Neimenggu population.Methods The method of DNA amplification with sequence-specific primers(PCR-SSP)was used to determine 17 alleles of HLA-DRB1*01,*04,*10 genotypes in 80 RA patients and 110 healthy controls from the Han nationality population in Neimenggu.Results The frequencies of SE were significantly increased in RA patiens com- pared with controls(48.8%20%,P＜0.01).Epitope analysis revealed that the most predominant allele subtype of DR4(*0405)was usceptible sequence in Neimenggu patients with RA(28.8%12%,P＜0.01).No statisticall significant difference of other subtypes of DR1,DR4 nd DR10 was noted including DRB1*0101(2.5%0.9%), *0102(2.5%0),*0103(1.25%0.9%),*0104(2.5%0),*0401(6.251.8%),*0402(3.75%0.9%),*0403 (1.25%1.8%),*0404(2.5%1.8%),*0406(2.5%2.7%),*0407(1.25%0.9%),*0408(3.75 %0.9%),*0409 (1.25%0),*0410(2.5%0.9%),*0411(00)and *1001(8.75%4.5%)respectively.Logistic regression analy- sis showed that the disease of patients with SE homozgote was more severe than that of patients with heterozy- gote(P＜0.01).Conclusion The results suggest that there is an association between SE of HLA-DRBI gene and susceptibility and severity of RA,especially,HLA-DR4 subtypes are strongly associated with RA in Han nationality in Neimenggu population.