HPLC Determination of Psoralen and Bergapten in Ficus hirta Vahl / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)1993.
Article
in Chinese
| WPRIM
| ID: wpr-683013
ABSTRACT
Objective To establish a HPLC method for the determination of psoralen and bergapten in Ficus hirta Vahl.Methods A Merck-lichrospher C18(4 mm?250 mm,5 ?m)column was adopted.The mobile phase was acetonitrile-water(35∶65) with the flow rate of 1.0 mL/min.The column temperature was 35 ℃,and the detection wavelength was set at 222 nm.Results Psoralen's linearity was obtained in the range of 0.12 ?g~1.20 ?g(r=0.999 7),and bergapten's linearity in the range of 0.03 ?g~0.30 ?g(r=0.999 5).Psoralen's average recovery was 100.9 %with RSD 2.9 %,and that of bergapten was 99.6 %with RSD 1.9 %.Conclusion This is the first report of simultaneous determination of psoralen and bergapten in Ficus hirta Vahl.by HPLC,and the results showed the method is accurate,reproducible,and can serve as quality evaluation method for Ficus hirta Vahl.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article
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