Inhibitory effect of erythromycin derivative on the expression of NF-KB in active T lymphocytes / 中华风湿病学杂志
Chinese Journal of Rheumatology
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-683194
ABSTRACT
Objective To elucidate the effects of erythromycin derivative,9(S)-hydroxyerythromycin, on the expression of NF-?B in active T lymphocytes,and evaluate the potential role in the treatment of rheuma- toid arthritis(RA).Methods Jurkat T cells were exposed to 100 pmol/L tumor necrosis factor(TNF)-?with or without pretreatment with 3,10,30 or I00?g/ml of erythromycin or 9(S)-hydrnxyerythromycin 1 h at 37℃. The expression of NF-?B was measured by reverse transcription-polymerase chain reaction(RT-PCR)and Western blot.Results Erythromycin and 9(S)-hydroxyerythromycin decreased the expression of NF-?B mRNA and protein in a dose-related fashion.The treatments with erythromycin(100?g/ml)and 9(S)-hydrox- yerythromycin(100?g/ml)resulted in about 36%,45% reduction of NF-?B mRNA(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Rheumatology
Year:
2003
Type:
Article
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