Spindle and chromosome configuration of human in vitro matured oocytes after slow freezing-fast thawing / 中华妇产科杂志
Chinese Journal of Obstetrics and Gynecology
; (12)2000.
Article
in Zh
| WPRIM
| ID: wpr-683385
Responsible library:
WPRO
ABSTRACT
Objective To evaluate the development of immature oocytes after freezing-thawing by conventional cryopreservation method for mature oocytes.Methods Immature oocytes were collected from stimulated ovaries of intracytoplasmic sperm injection(ICSI)cycles.Immature oocytes were in vitro matured directly or after slow freezing-fast thawing and immunostained for tubulin and chromatin and at last visualized by confocal microscopy.Results No statistical difference was found in maturity rate between freezing groups and the controls.There was a statistically significant increase in abnormalities of chromosome(23.7% vs. 50%)and spindle(28.9% vs.53.9%)in the GV freezing group compared with the GV control(P
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Obstetrics and Gynecology
Year:
2000
Type:
Article