CD4+ T Helper Cells Engineered to Produce IL-10 Reverse Allergen-induced Airway Hyperreactivity and Inflammation / 소아알레르기및호흡기학회지
Pediatric Allergy and Respiratory Disease
;
: 279-289, 2000.
Article
in Korean
| WPRIM
| ID: wpr-68359
ABSTRACT
PURPOSE:
Asthma is characterized by airway hyperreactivity to a variety of specific and nonspecific stimuli, by chronic airway inflammation with pulmonary eosinophilia, by mucus hypersecretion, and by increased serum IgE levels. T helper 2 (Th2) cells play a critical role in the pathogenesis of asthma, but the precise immunological mechanism that inhibit Th2 cell function in vivo are not well understood.METHODS:
Using gene therapy, Th-cell lines were transferred intravenously into histocompatible SCID or OVA immunized BALB/c mice. Airway responsiveness was assessed by methacholine-induced airflow obstruction from conscious mice placed in a whole-body plethysmograph. Pulmonary airflow obstruction was measured by enhanced pause (Penh).RESULTS:
We demonstrated that ovalbumin-specific (OVA-specific) Th cells engineered to express IL-10 abolished airway hyperreactivity induced by OVA-specific Th2 effector cells in SCID and BALB/c mice. The inhibitory effect of IL-10 transduced Th cells was antigen-specific and was reversed by neutralization of IL-10.CONCLUSION:
Our results demonstrate that IL-10 transduced CD4+ Th cells in the respiratory mucosa can indeed regulate Th2-induced airway hyperreactivity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovum
/
Pulmonary Eosinophilia
/
Asthma
/
Immunoglobulin E
/
Genetic Therapy
/
Interleukin-10
/
T-Lymphocytes, Helper-Inducer
/
Th2 Cells
/
Respiratory Mucosa
/
Inflammation
Limits:
Animals
Language:
Korean
Journal:
Pediatric Allergy and Respiratory Disease
Year:
2000
Type:
Article
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