THE CHEMICAL COMPOSITION AND SPECTRUM CHARACTERISTICS OF GLUCOAMYLASE FROM AN ASPERGILLUS NIGER MUTANT / 微生物学通报

ABSTRACT

Purified GAI, one form of glucoamylase obtained from Aspergillus niger mutant T21 contained 17.6% total carbohydrate. Analyses of amino acid composition showed that GAI contained about 25% Ser and Thr, 20.3% Asx and Glx and 6% basic amino acids. The UV absorption and fluorescence spectra of GAI were determined. The maximum absorption wavelength was at 278nm and minimum at 250nm. For fluorescent analyses the excitation and maximum emission spectra were at 284nm and 342nm respectively. The CD-spectrum determined showed two negative peaks. Its dispersion of secondary structure in solution showed 10.6% ?-helix, 16.3% ?-form and 73.1% unordered.