Effect of low dose irradiation on expression of membrane molecules of T lymphocytes from cord blood / 中国微创外科杂志

ABSTRACT

Objective To study the expression of membrane molecules of T lymphocytes of cord blood after low dose irradiation(LDI). Methods Freshly isolated lymphocytes from cord blood were irradiated with 62mGy gamma-ray. At different time(4h; 12h;24h) after irradiation, the changes of TCR+, CD3 +, CD4 +, CD8 + cells were examined by flow cytometry with direct immunofluorescence, respectively. Results The proportion of CD3 +, TCR+ /CD3 +; CD4 +, CD8 + cells increased significantly after LDI,the most obvious enhancement was noted in the 24h experimental group were no significant changes in the ratio of CD4 to CD8. Conclusions It is suggested that expedition of the maturation, activation and signal transudation of T lymphocytes from cord blood can be induced by ir- radiation with 62mGy gamma-ray The reconstruction of immune functions after cord blood transplantation may be ac- celerated, enhancing the graft versus leukemia(GVL)effect and preventing the tumor from relapsing.