Separation and purification of B-Phycoerythrin in Porphyridium cruentum / 中国海洋药物
Chinese Journal of Marine Drugs
;
(6)1994.
Article
in Chinese
| WPRIM
| ID: wpr-683881
ABSTRACT
The B Phycoerythrin(B PE) could both be separated after crude phycobiliproteins from Porphyridium cruentum were precipitated in ammonium sulphate and dialyzed,then chromatographed on a hydroxylapatite (HA) Column and a Sephadex G 100 column respectively.The purity of B PE separated by two methods was 4.92 and 3.78 (A 545 nm/A 280 nm) respectively.When the purified B PE was electrophoresed under polyacryiamide gradient gel electrophoresis condition,only one band was observed.The B PE possessed an absorption maximum at 545nm and a room fluoresence emission at 574.5nm.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Marine Drugs
Year:
1994
Type:
Article
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