Recombinant Kringle 5 of Human Plasminogen for Mammary Cancer Gene Therapy Mediated by Adenovirus / 中国肿瘤生物治疗杂志

ABSTRACT

Objective: To investigate the inhibitory effects of Ad K5 on the proliferation of endothelial cells and the growth of mammary cancer. Methods: Kringle 5 of human plasminogen was constructed by PCR, and then cloned to plasmid pCA13. Recombinant adenovirus Ad K5 was obtained through homologous recombination in 293 cells. The inhibition effect of Ad K5 on cell proliferation was observed in vitro . The tumor size was measured at different intervals to observe the antitumoral effect of Ad K5 in vivo .Results: The mRNA expression of K5 gene was detected in Ad K5 infected B Cap 37 cells. Ad K5 inhibited the growth of ECV 304 cells, but had almost no effects on B Cap 37 cells. It seemed that Ad K5 could inhibit endothelial cells but not kill the cancer cells directly. Ad K5 could also inhibit the tumor growth in vivo. Conclusion: Ad K5 could inhibit the growth of solid mammary tumor.