The growth-suppressive effect of retrovirus-mediated wild-type p53 gene on human esophageal cancer cell line / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-684167
ABSTRACT
Objectives:
To observe growth suppressive effects of human wild type p53(wt p53) gene on human esophageal cancer cell line.Methods:
Using the retroviral vector to introduce exogenous wt p53 gene into human esophageal cancer cell line ECA109,the gene expression and tumor inhibition were studied in vitro and in vivo .Results:
The expression of p53 in transfected cell lines(ECA 109/p53 ) was increased.The growth rates and the ability to form colony in soft agar were greatly inhibited in ECA 109/p53 cells versus ECA109/neo and ECA109 cells.The G 0+G 1 ratio increased and S ratio decreased in cell cycle distribution,and apoptosis index significantly rose in the ECA109/p53 cells,which were confirmed by FCM analyzing.The tumorigen icity of ECA109/p53 cells in nude mice was obviously suppressed by exogenous p53.Conclusions:
Exogenous wt p53 mediated by retroviral vector could inhibit the growth of human esophageal cancer cell.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article
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