Effects of post-burn plasma on signal molecule, NF-?B activation and interleukin 6 expression in cultured PBMC of rats / 医学研究生学报

ABSTRACT

Objectives:To study the alterations of calcium ion and NF ?B activation in cultured PBMC after stimulation of post burn plasma, and to investigate the roles of signal transduction pathway in the mechanisms of SIRS after burn. Methods:PBMC were stimulated with post burn plasma in vitro . The calcium ion concentration were measured by fura 2. The NF ?B protein were measured by EMSA. Results:Lots of NF ?B proteins were sequester in nucleus in all burn plasma groups. Dantrium and Nimodipine could decrease the activity of NF ?B protein in nucleus, and have significant inhibitory effects on the production of interleukin 6 and NO. Conclusions:In signal transduction levels, calcium ion may modulate the activity of NF ?B which mediate the expression of most proinflammtory cytokines.