Construction and identification of rGA antisense eukaryotic expression vector / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-684209
ABSTRACT
Objectives:
To construct rGA antisense eukaryotic expression vector.Methods:
Using molecular cloning technology, we subcloned rGA cDNA in its antisense orientations of the pcDNA 3.0 vector.Results:
Cutting with Eco RⅠ and Hin D Ⅲ,antisense recombinant plasmid produce 2 bands. One was 0.28 kb fragment, the other was 5.5 kb fragment which corresponded to theoretical calculation.Conclusions:
We succeeded in constructing rGA antisense eukaryotic expression vector.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article
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