Application of flow cytometry in sperm chromatin structure assay / 中华检验医学杂志

ABSTRACT

Objective To study the factors effecting on flow cytometric sperm chromatin structure assay.Methods The SCSA explores the metachromatic properties of acridine orange (AO) and flow cytometry to monitor the susceptibility of sperm chromatin DNA to acid induced denaturation in situ because of the low pH treatment. Results COMP?t, ?t, SD?t value were different upon on the storage methods (①sperm was preserved in 4℃, ②sperm was cryopreservated after dilution ③sperm was cryopreservated). Results show that the second method above made the least artificial injury to sperm DNA. It did not affect the results if the samples were quickly thawed in a 37℃ water bath and detected immediately and the detect current velocity was lower than 300 cell/sec. The intra -CV was 7 28% and the internal CV was 8 92%. Mean and standard deviations of COMP?t were 8 7?11 0% in 511 healthy men. Because data present right skew distribution, the reference range of COMP?t is