Cloning and Sequencing Na~+/H~+ AntiporterA Gene of Escherichia coli DH5? / 微生物学通报

ABSTRACT

The gene nhaA encodes functional protein that may play an important role in salt tolerance of Escherichia coli In order to study bacteria salt tolerance, a pair of primers were designed according to public nhaA sequence and was used to amplify 1 1kb DNA fragment with PCR The nhaA gene from E coli DH5?was cloned into a T vector and sequence analyses reveal that the cloned fragment contains entire nhaA gene coding region To apply the method of homology analysis,the result shows that many kinds of bacterium have nhaA gene, such as E coli K12, E coli O157H7, Salmonella typhimurium and Salmonella enterica , et al This analysis suggests that nhaA gene lie generally in bacterial; and it has intimate relation with salt tolerance of E coli that may be of great importance in improvement of the salt tolerance of plant