Application of two bacteria colony-PCR methods in the screening of phage antibody library / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-684635
ABSTRACT
Objective:
To value the application of two bacteria colony-PCR methods in the screening of phage antibody library.Methods:
Five positive monoclonal bacterium were respectively suspended in either deionized water or 0.1% Triton X-100, and then boiled to be used as template in PCR. . The DNAs products of PCR were extracted and digested by two enzymes, and then determined by electrophoresis.Results:
The inserted genes were detected in all the 5 clones after PCR and enzyme digestion .Conclusion:
Bacteria colony-PCR can be used in screening positive recombinant colonies. The bacteria colony-PCR method with bacteria colonies suspended in deionized water is valuable in large scale positive recombinant bacterium screening.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS