The Possible Relationship between Exercise-induced HSP72 mRNA and Ultrastructure of Myocardium in Rats / 中国运动医学杂志

ABSTRACT

Objective To research the possible relati onship between exercise-induced HSP72mRNA and ultrastructure of myocardium in rats. Methods 36 SD rats were randomly divided into four groupscontrol group,one week training group(T1),two weeks training group(T2),three weeks train ing group(T3).The rats in T1,T2 and T3 were trained on treadmill with 75%VO 2ma x intensity for 1 week,2 weeks and 3 weeks respectively.All rats were sacrificed within 24 hours after training and HSP72 mRNA expression of cardiac myocytes we re detected by reverse transcription-polymerase chain reaction(RT-PCR).The chang es of ultrastructure of myocardium in rats were also analyzed by electron micros cope.Results (1)HSP72 mRNA expression in T1 and T2 group were si gnificant higher than controls and T3 group.(2)According to electron microscope results of cardiac myocyte in rats,the sequence of damage degree from slight to severe was T3